Preclinical Evaluation of Robotic-Assisted Sentinel Lymph Node Fluorescence Imaging
Identifieur interne : 002671 ( Main/Exploration ); précédent : 002670; suivant : 002672Preclinical Evaluation of Robotic-Assisted Sentinel Lymph Node Fluorescence Imaging
Auteurs : Michael A. Liss [États-Unis] ; Salman Farshchi-Heydari [États-Unis] ; Zhengtao Qin [États-Unis] ; Sean A. Hickey [États-Unis] ; David J. Hall [États-Unis] ; Christopher J. Kane [États-Unis] ; David R. Vera [États-Unis]Source :
- Journal of nuclear medicine : official publication, Society of Nuclear Medicine [ 0161-5505 ] ; 2014.
Abstract
An ideal substance to provide convenient and accurate targeting for sentinel lymph node (SLN) mapping during robotic-assisted surgery has yet to be found. We used an animal model to determine the ability of the FireFly camera system to detect fluorescent SLNs after administration of a dual-labeled molecular imaging agent.
We injected the footpads of New Zealand White rabbits with 1.7 or 8.4 nmol of tilmanocept labeled with 99mTc and a near-infrared fluorophore, IRDye800CW. One and 36 h after injection, popliteal lymph nodes, representing the SLNs, were dissected with the assistance of the FireFly camera system, a fluorescence-capable endoscopic imaging system. After excision of the paraaortic lymph nodes, which represented non-SLNs, we assayed all lymph nodes for radioactivity and fluorescence intensity.
Fluorescence within all popliteal lymph nodes was easily detected by the FireFly camera system. Fluorescence within the lymph channel could be imaged during the 1-h studies. When compared with the paraaortic lymph nodes, the popliteal lymph nodes retain greater than 95% of the radioactivity at both 1 and 36 h after injection. At both doses (1.7 and 8.4 nmol), the popliteal nodes had higher (
The FireFly camera system can easily detect tilmanocept labeled with a near-infrared fluorophore at least 36 h after administration. This ability will permit image acquisition and subsequent verification of fluorescence-labeled SLNs during robotic-assisted surgery.
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DOI: 10.2967/jnumed.114.140871
PubMed: 25024425
PubMed Central: 4350927
Affiliations:
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Hickey</name><affiliation wicri:level="2"><nlm:aff id="A6">Department of Surgery, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Surgery, University of California, San Diego, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Hall, David J" sort="Hall, David J" uniqKey="Hall D" first="David J." last="Hall">David J. Hall</name><affiliation wicri:level="2"><nlm:aff id="A3">Department of Radiology, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Radiology, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A4">UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Kane, Christopher J" sort="Kane, Christopher J" uniqKey="Kane C" first="Christopher J." last="Kane">Christopher J. Kane</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Urology, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Urology, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A2">UCSD Moores Cancer Center. University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>UCSD Moores Cancer Center. 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Liss</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Urology, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Urology, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A2">UCSD Moores Cancer Center. University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>UCSD Moores Cancer Center. University of California, San Diego, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Farshchi Heydari, Salman" sort="Farshchi Heydari, Salman" uniqKey="Farshchi Heydari S" first="Salman" last="Farshchi-Heydari">Salman Farshchi-Heydari</name><affiliation wicri:level="2"><nlm:aff id="A3">Department of Radiology, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Radiology, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A4">UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Qin, Zhengtao" sort="Qin, Zhengtao" uniqKey="Qin Z" first="Zhengtao" last="Qin">Zhengtao Qin</name><affiliation wicri:level="2"><nlm:aff id="A3">Department of Radiology, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Radiology, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A4">UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A5">Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Hickey, Sean A" sort="Hickey, Sean A" uniqKey="Hickey S" first="Sean A." last="Hickey">Sean A. Hickey</name><affiliation wicri:level="2"><nlm:aff id="A6">Department of Surgery, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Surgery, University of California, San Diego, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Hall, David J" sort="Hall, David J" uniqKey="Hall D" first="David J." last="Hall">David J. Hall</name><affiliation wicri:level="2"><nlm:aff id="A3">Department of Radiology, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Radiology, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A4">UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Kane, Christopher J" sort="Kane, Christopher J" uniqKey="Kane C" first="Christopher J." last="Kane">Christopher J. Kane</name><affiliation wicri:level="2"><nlm:aff id="A1">Department of Urology, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Urology, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A2">UCSD Moores Cancer Center. University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>UCSD Moores Cancer Center. University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A6">Department of Surgery, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Surgery, University of California, San Diego, La Jolla</wicri:cityArea></affiliation></author><author><name sortKey="Vera, David R" sort="Vera, David R" uniqKey="Vera D" first="David R." last="Vera">David R. Vera</name><affiliation wicri:level="2"><nlm:aff id="A2">UCSD Moores Cancer Center. University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>UCSD Moores Cancer Center. University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A3">Department of Radiology, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Radiology, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A4">UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>UCSD In Vivo Cancer and Molecular Imaging Center, University of California, San Diego, La Jolla</wicri:cityArea></affiliation><affiliation wicri:level="2"><nlm:aff id="A6">Department of Surgery, University of California, San Diego, La Jolla, California</nlm:aff><country>États-Unis</country><placeName><region type="state">Californie</region></placeName><wicri:cityArea>Department of Surgery, University of California, San Diego, La Jolla</wicri:cityArea></affiliation></author></analytic><series><title level="j">Journal of nuclear medicine : official publication, Society of Nuclear Medicine</title><idno type="ISSN">0161-5505</idno><idno type="eISSN">1535-5667</idno><imprint><date when="2014">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">An ideal substance to provide convenient and accurate targeting for sentinel lymph node (SLN) mapping during robotic-assisted surgery has yet to be found. We used an animal model to determine the ability of the FireFly camera system to detect fluorescent SLNs after administration of a dual-labeled molecular imaging agent.</p><sec id="S1"><title>Methods</title><p id="P2">We injected the footpads of New Zealand White rabbits with 1.7 or 8.4 nmol of tilmanocept labeled with <sup>99m</sup>Tc and a near-infrared fluorophore, IRDye800CW. One and 36 h after injection, popliteal lymph nodes, representing the SLNs, were dissected with the assistance of the FireFly camera system, a fluorescence-capable endoscopic imaging system. After excision of the paraaortic lymph nodes, which represented non-SLNs, we assayed all lymph nodes for radioactivity and fluorescence intensity.</p></sec><sec id="S2"><title>Results</title><p id="P3">Fluorescence within all popliteal lymph nodes was easily detected by the FireFly camera system. Fluorescence within the lymph channel could be imaged during the 1-h studies. When compared with the paraaortic lymph nodes, the popliteal lymph nodes retain greater than 95% of the radioactivity at both 1 and 36 h after injection. At both doses (1.7 and 8.4 nmol), the popliteal nodes had higher (<italic>P</italic> < 0.050) optical fluorescence intensity than the paraaortic nodes at the 1- and 36-h time points.</p></sec><sec id="S3"><title>Conclusion</title><p id="P4">The FireFly camera system can easily detect tilmanocept labeled with a near-infrared fluorophore at least 36 h after administration. This ability will permit image acquisition and subsequent verification of fluorescence-labeled SLNs during robotic-assisted surgery.</p></sec></div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Californie</li></region></list><tree><country name="États-Unis"><region name="Californie"><name sortKey="Liss, Michael A" sort="Liss, Michael A" uniqKey="Liss M" first="Michael A." last="Liss">Michael A. Liss</name></region><name sortKey="Farshchi Heydari, Salman" sort="Farshchi Heydari, Salman" uniqKey="Farshchi Heydari S" first="Salman" last="Farshchi-Heydari">Salman Farshchi-Heydari</name><name sortKey="Farshchi Heydari, Salman" sort="Farshchi Heydari, Salman" uniqKey="Farshchi Heydari S" first="Salman" last="Farshchi-Heydari">Salman Farshchi-Heydari</name><name sortKey="Hall, David J" sort="Hall, David J" uniqKey="Hall D" first="David J." last="Hall">David J. Hall</name><name sortKey="Hall, David J" sort="Hall, David J" uniqKey="Hall D" first="David J." last="Hall">David J. Hall</name><name sortKey="Hickey, Sean A" sort="Hickey, Sean A" uniqKey="Hickey S" first="Sean A." last="Hickey">Sean A. Hickey</name><name sortKey="Kane, Christopher J" sort="Kane, Christopher J" uniqKey="Kane C" first="Christopher J." last="Kane">Christopher J. Kane</name><name sortKey="Kane, Christopher J" sort="Kane, Christopher J" uniqKey="Kane C" first="Christopher J." last="Kane">Christopher J. Kane</name><name sortKey="Kane, Christopher J" sort="Kane, Christopher J" uniqKey="Kane C" first="Christopher J." last="Kane">Christopher J. Kane</name><name sortKey="Liss, Michael A" sort="Liss, Michael A" uniqKey="Liss M" first="Michael A." last="Liss">Michael A. Liss</name><name sortKey="Qin, Zhengtao" sort="Qin, Zhengtao" uniqKey="Qin Z" first="Zhengtao" last="Qin">Zhengtao Qin</name><name sortKey="Qin, Zhengtao" sort="Qin, Zhengtao" uniqKey="Qin Z" first="Zhengtao" last="Qin">Zhengtao Qin</name><name sortKey="Qin, Zhengtao" sort="Qin, Zhengtao" uniqKey="Qin Z" first="Zhengtao" last="Qin">Zhengtao Qin</name><name sortKey="Vera, David R" sort="Vera, David R" uniqKey="Vera D" first="David R." last="Vera">David R. Vera</name><name sortKey="Vera, David R" sort="Vera, David R" uniqKey="Vera D" first="David R." last="Vera">David R. Vera</name><name sortKey="Vera, David R" sort="Vera, David R" uniqKey="Vera D" first="David R." last="Vera">David R. 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